Western blot analysis from lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL recombinant human IFN-γ and 20 ng/mL recombinant human TNF-a for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-human IL-36γ/IL-1F9 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46988) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for IL-36γ/IL-1F9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions.